Relatedness Among Plants as Measured by the DNA-Agar Technique.
نویسندگان
چکیده
An improved method for extraction of plant DNA is described. Quantitative species comparisons based on DNA-DNA hybridization are reported for several members of the family Leguminosae and for barley, wheat and rye. A maximum of about 10% homology in DNA polynucleotide sequences is found between monocotyledons and dicotyledons tested, whereas 20 to 90% homology is observed within a family. Species compared using a DNA fraction enriched for redundant polynucleotide sequences generally appear to be more closely related than when whole DNA is used. DNA-DNA hybridization may be useful in systematic and evolutionary study of plants, and also as a possible screening procedure for interfertility of species.
منابع مشابه
Relatedness among species of fungi and higher plants measured by DNA hybridization and base ratios.
HYLOGENETIC relatedness among organisms is reflected in their genotypes. The many efforts to compare genotypes have included comparisons of morphology, physiology, reproduction, and more recently ( MCCARTHY and BOLTON 1963; BOLTON 1966) the hybridization of DNA from different species and genera. These studie; have provided convincing evidence that it is possible to demonstrate relatedness, as j...
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ورودعنوان ژورنال:
- Plant physiology
دوره 42 7 شماره
صفحات -
تاریخ انتشار 1967